Composite

Part:BBa_K2088016

Designed by: Xia Chen   Group: iGEM16_NAU-CHINA   (2016-10-09)


nativeRBS-pbaA1A2B with a double Terminator

pbaA1A2B is responsible for the angular dioxygenation of 3-phenoxybenzoate(3-PBA). Complementation of pbaA1A2B results in the active 3-phenoxybenzoate 1',2'-dioxygenase, but the enzyme activity in Escherichia coli was achieved only through the coexpression of pbaA1A2B and a glutathione reductase (GR)-type reductase gene, pbaC.[1]

In this part, pbaA1A2 and pbaB are under their native RBS. Besides, this pba gene cluster is followed by a double terminator B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2648
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 116
    Illegal NgoMIV site found at 776
    Illegal NgoMIV site found at 1383
    Illegal NgoMIV site found at 1407
    Illegal AgeI site found at 1291
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None